The Next Generation Sequencing Unit, Node of the OMIC-Engine Research infrastructure at the Department of Molecular Biology & Genetics (MBG) of the Democritus University of Thrace (DUTH) in Greece is organizing a Five-day Hands-on Training School on Microbiome Sequencing & Data Analysis from 8-12 of October 2021.

The Training School will cover basic concepts of metagenomics and metatranscriptomics and will provide hands-on training on 16S/ITS metagenomic sequencing as well as analysis pipelines and data interpretation. The overall aim of the workshop is to give a working knowledge on microbiome sequencing and analysis that are integral to several fields of modern biology.

Target Audience

The Training School is open to students, researchers and technicians who wish to acquire an in-depth insight into the generation, analysis and interpretation of microbiome metagenomic sequencing data. M.Sc students /Ph.D. students/ postdoctoral fellows/technicians in academia or industry with an interest to work on microbiome analysis are encouraged to apply. The Five-day Hands-on Training School on Microbiome Sequencing & Data Analysis is supported by the OMIC-Engine Research infrastructure; therefore, participation is free of charge. OMIC-Engine is funded by the Operational program "Competitiveness, Entrepreneurship and Innovation" (NSRF 2014-2020).

Application Procedure

Interested participants should register for this workshop by filling up the application form here. The application deadline is September 8th, 2021. Fifteen candidates will be selected and notified by email until September 10th. Participants are welcome to bring and work with their own samples (1-2).

Learning outcomes

By the end of this course participants will be:
• familiar with different second and third generation sequencing methods
• able to design a microbiome sequencing approach for both prokaryotic and eukaryotic microbiome
• able to construct and quantitate libraries for NGS amplicon sequencing
• able to run and assess a sequencing experiment on the ION TORRENT platform
• able to analyze and interpret NGS microbiome sequencing data using ION Reporter, QIIME II and


Course registration and participation is FREE, thanks to the Action “Reinforcement of the Research and Innovation Infrastructure”, funded by the Operational Programme "Competitiveness, Entrepreneurship and Innovation" (NSRF 2014-2020) and co-financed by Greece and the European Union (European Regional Development Fund).

Language of instruction & material

The Training School will be conducted in English. Course material will be distributed to all accepted participants prior to course commencement.

Resources needed by the participants

For most of the course, specific open access software that is freely available on the Internet will be used, participants however will need to bring their own PC. Course material along with instructions will be distributed to the participants prior to course commencement.


The Training School is scheduled to run in physical presence. If this is not possible due to COVID-19 related restrictions, the training school will be held online.

Selection Criteria

Applicants will be selected based on their scientific background and research excellence. We also particularly encourage applications from researchers who might want to move into the field of Synthetic Biology. The organizers as well as the OMIC-Engine’s scientific committee, will collaborate to make the selection aiming to maximize diversity and inclusivity. All participants are asked to fully engage with the whole programme, enabling the best environment for networking, developing new collaborations and extending the state-of-the art for Synthetic Biology research. Spaces are limited (we have a maximum of 15 participants).

GDPR Compliance

OMIC-Engine’s project partners are subject to the EU General Data Protection Regulation (GDPR) which came into force on 25th May 2018. Data that is collected and processed for the purposes of facilitating and administering OMIC-Engine Training Schools is therefore subject to GDPR. Before registering please be sure to read the Data Protection Notice and the Privacy and Data Protection Policy before completing the Photo/Video Waiver form.